Coding

Part:BBa_K5002002:Design

Designed by: Siqi Chen   Group: iGEM23_Squirrel-Beijing-I   (2023-10-10)


Cellulose degrading enzyme,cep94A+cex+cenA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2966
    Illegal NotI site found at 3943
    Illegal NotI site found at 5087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 768
    Illegal BglII site found at 5000
    Illegal BamHI site found at 4136
    Illegal XhoI site found at 4498
    Illegal XhoI site found at 4747
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 846
    Illegal NgoMIV site found at 1942
    Illegal NgoMIV site found at 2599
    Illegal NgoMIV site found at 2972
    Illegal NgoMIV site found at 3474
    Illegal NgoMIV site found at 4278
    Illegal NgoMIV site found at 5203
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 108
    Illegal BsaI site found at 4182
    Illegal BsaI.rc site found at 3019
    Illegal SapI.rc site found at 3102


Design Notes

no


Source

no

References